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The MALDI-MS analysis of the following samples: (a) The assay mixture from compound 1 with SIRT1 and NAD+; (b) The assay mixture from compound 1 with NAD+ in the absence of SIRT1; (c) The assay mixture from NAD+ with SIRT1 in the absence of compound 1; (d) the MALDI matrix 3-hydroxypicolinic acid (3-HPA) only. All the MALDI-MS analyses were performed in a negative reflector mode. See ref. 67 for further experimental conditions.
