Figure 3.
MSC-plus-CsA administration prolongs rat islet allograft survival. (A) Islets (4,000 islet equivalents/rat) and allogeneic MSCs (2 × 106 cells/rat) were transplanted into recipient rats. (B) Islets (4,000 islet equivalents/rat) and autologous MSCs (2 × 106 cells/rat) were transplanted into recipient rat via the portal vein. Group 1 received donor islets without MSCs, group 2 received donor islets with MSCs, group 3 was treated with CsA (5 mg/kg/day) for 2 wks, and group 4 was treated with both MSCs and CsA (5 mg/kg) for 2 wks. *P < 0.001, group 4 versus groups 1, 2 or 3. POD, postoperative day. (C) Immunohistochemical staining for insulin (magnification ×200) and H&E (magnification ×200) in liver tissue from islet-transplanted rats on day 100 after transplantation. (D) Immunohistochemical staining for CD4 (magnification ×200) and CD8 (magnification ×200) in liver tissue from islet-transplanted rats on day 5 after transplantation.