MSC-plus-CsA administration increases the frequency of CD4+CD25+Foxp3+ T cells. CD4+ T cells were identified in the PE-fluorescence gate on the basis of the expression of CD4. Flow cytometry diagrams showed CD25 and Foxp3 positivity in CD4+ T cells from lymph node lymphocytes (A), splenocytes (B), PBMCs (C) and graft-infiltrating cells (D) of normal, rejected and surviving rats at 30 d. Plots representative of three experiments are shown. *P < 0.05, MSCs + CsA compared with rejected group. GIL, gaft-infiltrating lymphocytes.