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Induction of FGF21 mRNA levels with the β3-adrenergic agonist CL316243 is not hampered by coadministration of a PPARα antagonist. Differentiated brown adipocyte cells were treated with CL316243 for 6 h in the presence or absence of GW6471, and FGF21 mRNA levels were measured by quantitative RT-PCR. Data were analyzed by one-way ANOVA. Bars show means ± SEM of three independent experiments. Statistically significant differences between control and treated cells are denoted (*P < 0.01).
