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. 2011 Apr 20;17(7-8):619–627. doi: 10.2119/molmed.2010.00210

Figure 1.

Figure 1

VEGF/VEGFR2 status in CLL cells. (A) CLL cells secrete higher VEGF levels (235.9 ± 29.1 pg/mL) than PBMCs from healthy volunteers (94.1 ± 12.1 pg/mL) (P = 0.0002, Mann-Whitney test) as assessed by ELISA after 24 h of culture. (B) VEGF protein (i ) is present in cytoplasm of primary CLL cells. Also the presence of VEGFR2 (ii ), mainly localized to the membrane, and its phosphorylation (iii ) can be demonstrated by immunofluorescence. For all three antibodies, rabbit IgG antibody functioned as isotype (iso) control (iv). Magnification is 100×. One representative sample of six independent experiments is displayed. ***P < 0.001.