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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: Immunol Lett. 2011 May 20;139(1-2):25–32. doi: 10.1016/j.imlet.2011.04.011

Figure 1. TRAIL regulates the secondary expansion of helpless CD8+ T cells.

Figure 1

Untreated (helped) or CD4 depleted (helpless) C57BL/6J mice were immunized with 1×107 irradiated TAP−/− Ad5E1 MECs. At day 7 after immunization CD8+ T cells were purified and cultured for 6 days together with TAP+/+-Ad5E1 MECs cells in the absence or presence of Fas-Fc or DR5-FC (5ug/ml). The frequency of antigen-specific CD8+ T cells ex vivo, and after in vitro restimulation was determined by quantifying E1B192-200-specific IFN-γ producing cells by flow cytometry. Data of one representative mouse of three mice is shown. The frequency of IFN-γ producing cells within the CD8+ T cell population is depicted within the FACS plot. Numbers below the FACS plots show secondary expansion of E1B192-200-specific IFN-γ producing CD8+ T cells at the end of the culture as mean ± S.E.M. of 3–4 mice per group (representative of 4 experiments). *** p<0.005 compared to helped CD8+ T cells; * P<0.05 compared to helpless CD8+ T cells).