(a) Overlay of the signal responses achieved for each concentration of target miRNA. Concentrations utilized were 40 nM (black), 10 nM (red), 2.56 nM (green), 640 pM (blue), 160 pM (pink), 40 pM (cyan), 10 pM (orange), and a blank (with the exception of miR-16, which did not contain the 40 pM and 10 pM calibration points). (b) Calibration curves for the S9.6 response for miR-16, miR-21, miR-24-1, and miR-26a. Plots were constructed from the relative shifts at 40 min. The red curves represent the logistic fits to the data points. Error bars represent ±1 standard deviation for between 4 and 12 independent measurements at each concentration.