Fig. 3.

Metabolism of nucleotides in highly enriched astroglial cultures (a, c, e) and co-cultures (b, d, f). Cultures were incubated with 0.1 mM of ATP, ADPβS or UTP, and samples were collected at 0, 1, 3, 8, 24 and 48 h. Nucleotides and their metabolites were quantified by HPLC-UV as described in Materials and methods. Values are means ± SEM from four experiments. *P < 0.05, significant differences from highly enriched astroglial cultures