Fig. 4.
Representative Western blots showing P2Y1, P2Y6 and P2Y12 receptors expression in highly enriched astroglial cultures (AC) and co-cultures (CC). Total P2Y1 (tP2Y1), P2Y6 (tP2Y6) and P2Y12 (tP2Y12) receptors and actin (43 kDa) expression were obtained from whole cell lysates. Expression of membrane P2Y1 (mP2Y1) and P2Y6 (mP2Y6) receptors was obtained from surface biotinylated and immunoprecipitated proteins. Three immunoreactive bands of 42, 90 and 266 kDa specifically reacted with rabbit anti-P2Y1 antibody, and those of 25, 36 and 86 kDa specifically reacted with rabbit anti-P2Y6 antibody. These bands were absent in the presence of the respective neutralising peptides. A single band of ~65 KDa was detected with the rabbit anti-P2Y12 antibody