Fig. 2.

Expression of P2Y₂ receptors and co-localization with pituitary hormones in the anterior pituitary of the rat. a Merged image of P2Y₂ receptor-immunoreactive (ir) (red) and ACTH-ir (green) cells. Note that there was no colocalisation between P2Y₂ receptor- and ACTH-ir cells. b Merged image of P2Y₂ receptor-ir (red) and FSH-ir cells (green). Note that there was no colocalisation between P2Y₂ receptor- and FSH-ir cells. c Merged image of P2Y₂ receptor-ir (green) and GH mRNA stained (red) cells. Note that no colocalised cells with P2Y₂ receptors and GH mRNA signals are observed. d Merged image of P2Y₂ receptor-ir (red) and PRL-ir cells (green). Note that some P2Y₂ receptor-ir cells are also immunoreactive for PRL. One of the double-labeled cells (yellow in color) is indicated by a yellow arrow. Single-labeled FSH-ir and P2Y₂-ir cells are also observed. A pink arrow and a blue arrow indicate a single labeled FSH-ir and P2Y₂-ir cell, respectively. e Merged image of P2Y₂-ir and TSH-ir cells. Note that there is no colocalisation. f a merged image of P2Y₂ receptor-ir (red) and S100-ir (green) cells. Note that almost all of the S100-ir cells are also labeled with P2Y₂ immunoreactivity, but a proportion of the P2Y₂-ir cells are not labeled by S100 antibody, such as the one indicated by a red arrow. A yellow arrow indicates a double-labeled cell. All scale bars = 60 μm