Fig. 6.

Results of Western blotting (WB) and control experiments. a WB results. The color ladder is the molecular marker of protein; lanes 1, 3, 5, 7, 8, 9 are the results of P2Y₁, P2Y₂, P2Y₄, P2Y₆, P2Y₁₂, and P2Y₁₃ WB, respectively. Note that immunoreactive bands were detected at different molecular weight levels: one band for P2Y₁ receptors at about 48 kDa (lane 1), two bands for P2Y₂ receptors at about 36, 90 kDa (lane 3), one band for P2Y₄ at about 49 kDa (lane 5), no clear band for P2Y₆ or P2Y₁₂ receptors (lane 7, lane 8), one band for P2Y₁₃ at about 37 kDa (lane 9); lanes 2, 4, 6 and 10 are the results of P2Y₁, P2Y₂, P2Y₄, and P2Y₁₃ peptide pre-absorption experiments. Note that pre-adsorption of the antiserums with the peptide antigens resulted in the absence of bands (lanes 2, 4, 6, 10). Control experiments were carried out with the P2Y antibodies pre-absorbed with their respective peptides. No staining was observed in those preparations incubated with antisera solutions pre-absorbed with their respective peptides, as shown for P2Y₂ and P2Y₄ receptor antisera (b, c). Scale bars = 60 μm