Fig. 2.

(A) Intracellular translocation of PKCδ following Wnt-4 treatment. Cytosolic and membrane proteins were separated from control, Wnt-4 treated and Wnt-4 overexpressing Tep1 cells. Western blot analysis demonstrated PKCδ translocation. Loading controls are shown below the Western blot as Ponceau red stained total protein. Representative blots and stainings are shown from three repeats. (B) Changes of PKCδ expression with age by Q-RT-PCR. TECs were purified from young (1 month) and ageing (9 months) mice. Data were normalized to β-actin housekeeping gene. Statistically significant differences are marked by asterisks. (C and D) Age associated changes in PKCδ expression by histology. Cryostate sections of 1 month and 9 months old mouse thymi were stained with anti- PKCδ− NL663, anti-EpCAM1-FITC and anti-Ly51-PE. The overlay of the staining pattern is also shown. Characteristic stainings are shown from a minimum of five repeats.