Fig. 4.

Analysis of Fz-4, Fz-6, Dvl and PKCδ co-localisation. (A and B) Western blot analysis following immunoprecipitation using anti-Fz-6-antibody (A) or anti-Fz-4-antibody (B) shows increased association of Fz-6 with PKCδ and Dvl upon Wnt-4 treatment. Representative blots are shown from two repeats. (C and D) Histology of 1 month and 9 months old mouse thymi using anti- PKCδ– NL663, anti-Fz-6-NL557 and anti-EpCAM1-FITC antibodies. The overlay of the three stainings is shown. Size markers are shown in the corner of all histology figures. Characteristic stainings are shown from a minimum of five repeats.