Table 2.

Effects of TFE on folding properties of WT and ΔN-KcsA in a PC:PA (7:3 mol%) lipid bilayer

Sample	Wavelength (nm)a					K SV (M−1)b
TFE (vol%)	0	5	10	20	30	0	5	10	20	30
WTc	320	320	320	321.5	321.5	3 ± 0.6	2.6 ± 0.8	2.5 ± 1.2	2.7 ± 0.8	8.0 ± 1.8
∆Nd	320	320	320	320	320	2.2 ± 0.7	2.8 ± 1	4.5 ± 0.8	6.5 ± 2.3	7 ± 1.6

^aParameters were derived from Trp fluorescence data shown in Fig. 3b

^bThe Stern–Volmer quenching constants were derived from the slopes of the linear regression lines from plots of F ₀ /F = 1 + K _SV [Q]. All values are the means ± SD of three experiments

^cThe values for WT in PC:PA (7:3) were derived from the previously published work (Raja et al. 2007)

^dThe values were derived from Trp fluorescence emission spectra and acrylamide quenching plots (data not shown)