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. 2011 Apr 21;4(4):759–770. doi: 10.1093/mp/ssr033

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© The Author 2011. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 2. — Specific Amino Acid Substitutions in ASP2 Suppress rus Phenotype.

(A) Amino acid sequence from 247–296 of the ASP2 protein with the position and nature of amino acid changes shown for each of the suppressor alleles. Arrow indicates the lysine residue that binds to pyridoxal-5'-phosphate (PLP). Human cytosolic AAT (HsGOT1) and Arabidopsis ASP1, ASP3, and ASP5 are shown for sequence comparison.

(B) Complementation of asp2 suppression alleles by WT Arabidopsis ASP2 and Human GOT1. Phenotypes of rus1-2 asp2-12 (r1-2 a2-12) plants expressing either the Arabidopsis ASP2 cDNA (ASP2) or the human cytosolic AAT cDNA (Hs-cGOT1) under the Cauliflower Mosaic Virus 35S promoter (35S) are shown. Bar = 0.5 cm.

(C) Suppression of rus single and double mutants. Phenotypes of asp2-12 (a2-12) suppressor mutation either singly or in combination with rus2-1 (r2-1) or rus1-2 rus2-1 (r1-2 r2-1) are shown. Bar = 0.25 cm.

(D) Root-length measurements of 10-day-old seedlings shown in (C). Root lengths of single (a2-12), double (r2-1 a2-12; r1-2 r2-1), and triple (r1-2 r2-1 a2-12) mutant seedlings (n = 9 or 10) were measured. Error bar = standard error.