Skip to main content
. 2011 Jun 15;96(8):2502–2511. doi: 10.1210/jc.2011-0055

Fig. 1.

Fig. 1.

CCL2 is the most potent chemoattractant for THP-1 cells and MO secreted by IL-1β- or TNF-α-stimulated first trimester decidual cells. The relative chemotaxis in THP-1 cells (A and C) or MO (B and D) by chemokines produced by IL-1β- or TNF-α-stimulated first trimester decidual cells, was evaluated by incubation with or without (Con) excess each individual neutralizing antibody to CCL2, CCL4, CCL5, CCL7, CXCL8, or antibody cocktail (All). Cells (5 × 105) placed in upper chambers of 24-well plates were allowed to migrate into antibody-treated CM for 3 h. Migrated cells were then collected and counted with FACSCalibur using CellQuest software. The results were shown as the percentage inhibition by neutralizing antibody. *, P < 0.05; **, P < 0.01, each group vs. Con. #, P < 0.05; ##, P < 0.01, vs. anti-CCL2. Data are shown as mean ± sem (n = 8).