Fig. 2.
CCL5 is the most potent chemoattractant for iDC secreted by IL-1β- or TNF-α-stimulated first trimester decidual cells. A, The expression of DC maturation markers in DC treated with or without LPS. The relative chemotaxis in iDC by chemokines produced by IL-1β- (B) or TNF-α (C)-stimulated first trimester decidual cells was evaluated by placing iDC (1 × 105) in the upper chambers of 24-well plates and migrating iDC into CM treated with or without (Con) antibody for 5 h. The results were shown as the percentage inhibition by neutralizing antibody. *, P < 0.05; **, P < 0.01, each group vs. Con. #, P < 0.05; ##, P < 0.01, vs. anti-CCL5 or anti-CCL7. &, P < 0.05 vs. anti-CCL20. Data are shown as mean ± sem (n = 8).