Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Nucleotide sequence of primers and probe used in pGEM-T Easy cloning and qRT-PCR assay.
| Assay | Primer/Probe | Sequence (5'→ 3') | Nucleotide positions |
|---|---|---|---|
| qRT-PCR | Forward Primer | TACGGCGTGCAGTGCTTCT | 196-214 |
| Reverse Primer | AGATGGTGCGCTCCTGGAT | 277-295 | |
| TaqMan Probe | CTACCCCGATCACATGAAGCAGCACG | 219-244 | |
| Cloning | Forward Primer | AAGTTCATCTGCACCAC | 133-149 |
| Reverse Primer | TGTAGTTGCCGTCATCCT | 302-319 | |
