Table 1.
Primers used in this study
| Gene and primer | Positions* | Sequence (5'-3') |
|---|---|---|
| sabR | ||
| sab1-F | +38 to +57 | CATATGGCTCAGCAGGACCGAGC |
| sab1-R | +697 to +681 | CTCGAGGCAGGCGATGCCCGACA |
| sab2-F | +38 to +57 | CATATGGCTCAGCAGGACCGAGC |
| sab2-R | +700 to +681 | GGATCCTCAGCAGGCGATGCCCGACA |
| ER-F | -132 to -115 | CCCCGTGGCACCGTTCAT |
| ER-R | +143 to +160 | GACACCGGCCCGCTTGAG |
| S1R-F | -457 to -440 | GCCAGAGCCGACACCACA |
| S1R-R | +44 to +60 | ACGGCTCGGTCCTGCTG |
| sanG | ||
| Gare1-F | -1035 to -1016 | GGAATTCCGCCCGGCAGCAGCTGGACT |
| Gare1-R | -62 to -85 | GGGGTACCAAGTGGCTCCATGTATCCGCGACC |
| Gare2-F | -34 to -12 | GCTCTAGACCAGCTCAGGAGAATGCTCGATA |
| Gare2-R | +772 to +753 | CCCAAGCTTCTCCGCGACCTCGTCATCAT |
| EG0-F | +1404 to +1423 | AGGCCACCCTGCAGACGTAC |
| EG0-R | +1741 to +1724 | GAGGAGCGTGTCGGCTTG |
| EG1-F | -415 to -396 | GCGGGAGAACGTCACCTGTT |
| EG1-R | +104 to +86 | CGGGTCGGCTGTGGTGAGT |
| EG2-F | +340 to +360 | GCTCCGAGACCGTGACGAAAG |
| EG2-R | +763 to +743 | CTCGTCATCATCAGCGTGGGT |
| EG3-F | +743 to +763 | ACCCACGCTGATGATGACGAG |
| EG3-R | +1218 to +1198 | GACGCGGTTTGTTGCTCTTGA |
| RealG-F | +1405 to +1423 | GGCCACCCTGCAGACGTAC |
| RealG-R | +1536 to +1519 | CGGGACAGGTCGAACGTG |
| sanN and sanO | ||
| ENO-F | -114 to -98 | TCTTGGTCGCCAGGTCC |
| ENO-R | +135 to +153 | CTTCGGATGCTGAATGTGC |
| sanF | ||
| EF-F | -267 to -250 | CGCGCAGGTCGGCCAGGT |
| EF-R | +234 to +213 | TACTGCTTCTCGTGCTTCGGGT |
| RealF-F | +70 to +87 | GGTGCTGACGCTCGACTC |
| RealF-R | +257 to +238 | TGAGGTCCACGAGGTTCATC |
| hrdB | ||
| S1H-F | -820 to -803 | GGGTACGCCCCGTCAGTG |
| S1H-R | +241 to +224 | AGCCTTTCCCCGCTCAAT |
| RealH-F | +290 to +308 | ACTGAGTGGCCGGAATCTG |
| RealH-R | +225 to +206 | GTCTCTGTCATGGCGCTCAT |
* The nucleotide positions for sanG, sanN, sanO, sanF, sabR and hrdB are given with respect to their transcription start points as +1. The enzyme recognition sites are underlined in the primer sequence.