Table 2.
Strains and plasmids used in this study.
| Strain | Description and genotype | Sources/References |
|---|---|---|
| Shewanella oneidensis | ||
| MR-1 | Wild type strain | BCC/[2] |
| luxS-ins | pKnock-Km plasmid inserted into the luxS gene | this study |
| WTKm | pKnock-Km plasmid inserted before the luxS gene | this study |
| Escherichia coli | ||
| S17-1λpir | Biparental mating TpR SmR recA thi pro hsdR RP4-2-Tc::Mu-Km::Tn7 λpir |
Biomedal (Spain)/[29] |
| pir-116 | Maintaining of R6K ori plasmids F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHFR) |
Epicentre/[30] |
| DH5α | Subcloning routine F- Φ80lacZΔM15 Δ(lacZYA-argF)U169 recAl endAI hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relAI λ- |
Invitrogen/[31] |
| HB101 | Carrier of the pRK2013 plasmid for triparental mating. F- supE44 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 Δ(mcrC-mrr) recA13 rpsL20 thi-1 Δ(gpt-proA)62 hsdSB20 λ- |
[32] |
| Vibrio harveyi | ||
| BB152, BAA-1119 | luxM::Tn5; produces only AI-2 | ATCC/[33] |
| MM77 | luxM::Tn5, luxS::CmR; produces neither AI-1 nor AI-2 | ATTC/[34] |
| BB170, BAA-1117 | luxN::Tn5; senses only AI-2 | ATCC/[33] |
| Plasmids | ||
| pKnock-Km | KmR; oriT; source plasmid for the suicide vectors into Sh. oneidensis | NCCB 3407/[24] |
| pEX18Ap | AmpR; source plasmid for a replicating plasmid into Sh. oneidensis | Max Schobert, TU Braunschweig/[35] |
| pPS858 | AmpR, GmR; source of the Gentamicin-GFP (Gm-GFP) cassette | Max Schobert, TU Braunschweig/[35] |
| pRK2013 | KmR; helper plasmid for conjugation | DSMZ 5599/[36] |
| pKnock-luxS | Construction of luxS-ins KmR; pKnock-Km containing an internal fragment of luxS of Sh. oneidensis |
This study |
| pBA2106 | Construction of WTKm KmR, pKnock-Km containing an upstream fragment of luxS of Sh. oneidensis |
This study |
| pEX18ApGm | For tagging Sh. oneidensis with gfp KmR, GmR, the Gm-GFP cassette ligated into pEX18Ap |
This study |