Table 2.
totalRNA yield in ng
| Number of Gyrodactylus salaris individuals | 1 | 10 | 100 | average per individual | ||||
|---|---|---|---|---|---|---|---|---|
| totalRNA Extraction Kit | yield SD | Quality SD | yield SD | quality SD | yield SD | quality SD | yield SD | quality SD |
| Trizol Plus | 2.8 ± 0.8 | 2.6 ± 1.1 | 19.0 ± 2.2 | 9.6 ± 0.4 | 212.8 ± 50.6 | 9.9 ± 0.1 | 2.3 ± 0.4 | 7.4 ± 4.1 |
| Molestrips totalRNA basic | 5.0 ± 0.9 | 8.3 ± 0.2 | 75.7 ± 14.2 | 10.0 ± 0.1 | 562.7 ± 245.2 | 9.4 ± 1.3 | 6.1 ± 1.4 | 9.2 ± 0.9 |
| mirVana | 7.8 ± 0.6 | 6.7 ± 1.3 | 91.1 ± 11.6 | 9.4 ± 0.9 | 410.9 ± 53.8 | 9.7 ± 0.5 | 7.0 ± 2.6 | 8.6 ± 1.6 |
| ZymoResearch RNA miniprep | 20.7 ± 2.4 | 2.7 ± 0.3 | 74.0 ± 5.2 | 3.0 ± 0.1 | 576.3 ± 124.9 | 4.0 ± 0.5 | 11.3 ± 8.2 | 3.2 ± 0.7 |
| Phenol-free Total RNA Purification | 15.4 ± 6.1 | 9.2 ± 1.2 | 142.0 ± 27.8 | 9.9 ± 0.2 | 1908.5 ± 500.6 | 9.5 ± 0.9 | 16.2 ± 2.5 | 9.5 ± 0.4 |
| MasterPure Complete RNA Purification | 25.8 ± 4.3 | 2.3 ± 0.2 | 158.4 ± 25.9 | 3.2 ± 0.5 | 505.0 ± 59.7 | 6.2 ± 0.7 | 15.6 ± 10.4 | 3.9 ± 2.0 |
| average absolute | 12.9 ± 9.2 | 5.3 ± 3.1 | 93.4 ± 50.6 | 7.5 ± 3.4 | 696.0 ± 608.9 | 8.1 ± 2.4 | 9.7 ± 5.6 | 7.0 ± 2.7 |
| average per individual | 12.9 ± 9.2 | 5.3 ± 3.1 | 9.3 ± 5.1 | 7.5 ± 3.4 | 7.0 ± 6.1 | 8.1 ± 2.4 | ||
TotalRNA yield and quality of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RNA-quality is expressed as RQI/RIN values determined by the software following the Experion and 2100 Bioanalyzer electrophoresis systems respectively. The global average over all methods and sample sizes is depicted in bold.
SD-standard deviation