Table 3.
small/miRNA yield in ng
| Number of Gyrodactylus salaris individuals | 1 | 10 | 100 | average per individual | ||||
|---|---|---|---|---|---|---|---|---|
| totalRNA Extraction Kit | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD |
| Trizol Plus | 4.3 ± 4.5 | 1.1 ± 0.9 | 8.2 ± 11.2 | 2.5 ± 3.6 | 22.9 ± 29.6 | 3.5 ± 5.0 | 1.8 ± 2.2 | 0.5 ± 0.6 |
| Molestrips totalRNA basic | 4.2 ± 4.7 | 0.5 ± 0.7 | 10.8 ± 15.1 | 2.5 ± 3.5 | 13.6 ± 8.8 | 0.9 ± 0.7 | 1.8 ± 2.1 | 0.2 ± 0.2 |
| mirVana | 1.3 ± n.a. | 0.4 ± n.a. | 44.4 ± 39.9 | 6.1 ± 5.6 | 214.2 ± 81.7 | 22.4 ± 9.6 | 2.6 ± 1.6 | 0.4 ± 0.2 |
| ZymoResearch RNA miniprep | 22.3 ± 13.8 | 9.6 ± 4.8 | 70.0 ± 28.5 | 26.6 ± 9.4 | 333.4 ± 112.0 | 51.6 ± 16.4 | 10.9 ± 10.1 | 4.3 ± 4.7 |
| Phenol-free Total RNA Purification | 1.8 ± n.a. | 0.2 ± n.a. | 56.9 ± 56.6 | 7.8 ± 7.6 | 389.3 ± 261.0 | 33.7 ± 22.0 | 3.8 ± 2.0 | 0.4 ± 0.3 |
| MasterPure Complete RNA Purification | 19.7 ± 10.0 | 9.2 ± 3.6 | 103.4 ± 36.5 | 25.9 ± 9.0 | 207.7 ± 47.1 | 41.1 ± 8.7 | 10.7 ± 8.8 | 4.1 ± 4.6 |
| average absolute | 8.9 ± 9.5 | 3.5 ± 4.6 | 49.0 ± 36.4 | 11.9 ± 11.3 | 196.9 ± 154.9 | 25.6 ± 20.5 | 5.3 ± 4.3 | 1.7 ± 2.0 |
| average per individual | 9.7 ± 9.2 | 3.9 ± 4.4 | 4.9 ± 3.6 | 1.2 ± 1.1 | 2.0 ± 1.5 | 0.3 ± 0.2 | ||
SmallRNA and microRNA yield of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimen. The global average over all methods and sample sizes is depicted in bold.
SD-standard deviation