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LPS induction of primary human chondrocytes. Release of A, IL-6 and IL-8; C, nitrate; and D, PGE2 from cultured human chondrocytes treated with 0-1000 ng/ml LPS for 48 hours. B, Dose dependent inhibition of LPS (1000 ng/ml) induced IL-6 release by polymyxin-B (PMB). PMB was added at 3 μg/ml in C and D. The error bars indicate SD of averaged treatment results (duplicate wells) from 3 donors.
