Figure 6.
Effects of ECI-(GlcNAc)2 or ECI-[(Man)3(GlcNAc)2] on MMP-2 production by fibroblasts. A, After culture of fibroblasts in the presence of synthetic ECI-(GlcNAc)2 or ECI-[(Man)3(GlcNAc)2] for 3 days, the conditioned media were subjected to enzyme immunoassay. Data are mean ± SEM (n = 3). Control, solvent control for ECI-(GlcNAc)2 and ECI-[(Man)3(GlcNAc)2]. *p < 0.01, compared with the control. B, The conditioned media obtained as described above were subjected to gelatin zymography. At the same time, conditioned media from co-culture experiments of FU-EPS-1 tumor cells and fibroblasts were also applied to the same gel. Fb, fibroblasts. Fb control, fibroblast cultures with solvent control for ECI-(GlcNAc)2 and ECI-[(Man)3(GlcNAc)2].