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. 2011 Jul 19;11:303. doi: 10.1186/1471-2407-11-303

Figure 1.

Figure 1

CM derived from GBM cell lines LN18 and LN229 enhances angiogenic and mitogenic capabilities of HUVEC. A) Tube-like formation assay was performed in SFM or LN18 and LN229-derived CM mixed (1:1) with VCBM for 24 h, as described in Materials & Methods. The graph shows relative % increase of ES formed by HUVEC with the number in SFM taken as = 100%. Columns, mean of at least three independent experiments done in triplicates; bars, SE. ** P < 0,001 (vs. control SFM: VCBM). B) Representative phase contrast pictures (magnification 10×) of HUVEC in vitro angiogenesis in response to LN18 or LN229 CM for 24 h. SFM:VCBM treatment was used as control. The cultures were evaluated and photographed as described in Materials and Methods. C) HUVEC were grown in SFM:VCBM or LN18 and LN229-derived CM mixed (1:1) with VCBM for 24 or 48 h. The graph represents relative growth increase with SFM:VCBM taken as reference (0%). Columns, mean of at least three independent experiments done in triplicates; bars, SE. * P < 0,05 and ** P < 0,001 (vs. control SFM:VCBM).