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GBM cells express leptin and VEGF. A) The abundance of leptin and VEGF mRNA was studied with qRT-PCR as described in Materials and Methods. The graphs represent leptin and VEGF mRNA levels relative to LN18 levels in SFM (= 1) ± SD. B) Leptin and VEGF secreted protein levels were measured in LN18 and LN229 CM by ELISA, as described in Materials and Methods. The values represent pg/mL per 9 × 106 LN18 cells and 6 × 106 LN229 cells ± SD.
