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Michaelis-Menten kinetic constants of laccase CLEAs for the oxidation of ABTSa.
| Sample | Km (mM) | Kcat (μmol/s/mg) | Kcat/Km (L·mg/s) |
|---|---|---|---|
| CLEA-1.0-136 | 0.083 ± 0,015 | 30.760 ± 0.900 | 0.369 |
| CLEA-1.5-100 | 0.156 ± 0,026 | 2.524 ± 0.091 | 0.016 |
| CLEA-1.867-50.5 | 0.259 ± 0,044 | 0.986 ± 0.046 | 0.004 |
| CLEA-1.0-50.5 | 0.101 ± 0,027 | 5.295 ± 0.250 | 0.053 |
| Free laccase | 0.082 ± 0,010 | 2.694 ± 0.060 | 0.033 |
aResults are mean of triplicate measures ± standard deviation.
