Table 2.

Immobilization parameters for Lipase G on different supports and protocols.

Immobilization protocols	Support	Activating agent	IP (mg·g−1)	HA (IU·g−1)	RA (%)
Ionic adsorption	MANAE-agarose*	none	4.52 ± 0.18	55.6 ± 2.60	14.2 ± 0.40
covalent attachment	Glyoxyl-agarose*	glycidol	4.30 ± 0.20	0	0
	MANAE-agarose*	glutaraldehydea	4.47 ± 0.12	0	0
	MANAE-agarose*	glutaraldehydeb	4.52 ± 0.18	31.2 ± 4.50	8.20 ± 0.80
	MANAE-agarose*	glutaraldehydec	4.05 ± 0.05	0	0
	MANAE-agarose*	glutaraldehyded	4.09 ± 0.08	0	0
	Epoxy-SiO2-PVA*	epichlorohydrind	3.00 ± 0.04	9.50 ± 0.30	4.00 ± 0.20
	Epoxy-SiO2-PVA**	epichlorohydrin	2.50 ± 0.02	128.2 ± 8.10	60.9 ± 5.30

IP: Immobilized protein; HA: hydrolytic activity; RA: recovered activity.

*Immobilization in aqueous medium.

**Immobilization in organic medium (hexane).

^aCovalent attachment in 100 mmoL·L⁻¹ buffer phosphate at pH 7.0.

^bDerivative prepared by ionic adsorption cross-linked with glutaraldehyde solution.

^cCovalent attachment in 100 mmoL·L⁻¹ buffer Tris-HCl at pH 7.0.

^dCovalent attachment in 2.5 mmoL·L⁻¹ buffer Tris-HCl at pH 7.0.