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Immobilization parameters for different lipase loadings offered to MANAE-agarose.
| Protein loading (mg·g−1 of support) | IP (mg·g−1) | HA (IU·g−1) | RA (%) |
|---|---|---|---|
| 5 | 4.52 ± 0.18 | 55.6 ± 2.60 | 14.2 ± 0.40 |
| 10 | 6.80 ± 0.50 | 59.2 ± 2.80 | 10.4 ± 0.60 |
| 15 | 9.38 ± 0.21 | 66.7 ± 4.60 | 8.45 ± 0.50 |
| 20 | 9.31 ± 0.16 | 68.8 ± 2.70 | 8.80 ± 0.20 |
| 30 | 9.23 ± 0.10 | 63.8 ± 4.60 | 8.20 ± 0.50 |
IP: immobilized protein; HA: hydrolytic activity; RA: recovered activity.
