Skip to main content
. 2011 Apr 6;31(14):5353–5364. doi: 10.1523/JNEUROSCI.0282-11.2011

Figure 9.

Figure 9.

EphB TKO brains exhibit reduced synaptic expression of NR2A and NR2B. A, Lysates from brains of WT, EphB DKO, and EphB TKO animals were fractionated to isolate supernatant (S1), crude membrane (P1), and pure synaptosome (Syn) fractions. Western blots were probed with indicated antibodies and show enrichment of glutamate receptor subunits in the synaptosome fraction of all animals. PSD-95 and EphB2 are enriched in the same fraction. B–E, Syn/P1 ratio was used to compare synaptic versus nonsynaptic expression of NMDA and GluR2 glutamate receptors subunits. Compared with the WT and EphB DKO brains, EphB TKO animals exhibit reduced synaptic expression of NR2A (C) and NR2B (D) subunits of the NMDAR. There is no change in synaptic expression the GluR2 subunit (E) of AMPA receptors between different genotypes. ANOVA, *p < 0.005; n = 3 animals for each condition. Error bars indicate SEM.