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. 2011 Jul;191(2):405–417. doi: 10.1111/j.1469-8137.2011.03693.x

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Copyright © 2011 New Phytologist Trust

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Fig. 6 — Nitric oxide (NO) measurement and nodulation phenotype on transgenic hairy roots overexpressing the Sinorhizobium meliloti Hmp flavohemoglobin under the control of the nodule specific promoter pMtENOD20. (a) Representative confocal microscopic pictures obtained from observation with DAF-2DA probe of transgenic Hmp-overexpressing and control glucuronidase (GUS) nodules slices (11 d post-inoculation). Bar, 100 μm. (b) Quantification of the NO levels by spectrofluorimetric method. The Hmp-overexpressing and control (GUS) transgenic roots were incubated with nonpermeant DAF2 probe to measure the NO produced and released in the medium. Results presented from roots 5 dpi. n=68 and n=62 for pMtENOD20-GUS (tinted bar) and pMtENOD20-hmp (open bar), respectively. Asterisk indicates a statistically significant difference (Student's t-test, P<0.05). (c) Number of nodules induced by the wild-type S. meliloti strain on transgenic Hmp-overexpressing and control hairy roots measured 11 d post-inoculation. Three independent experiments were done with a number of plants, n=78 for pMtENOD20-GUS (tinted bar), and n=69 for pMtENOD20-hmp (open bar). Asterisk indicates a statistically significant difference (Student's t-test, P<0.05).