Fig. 5.

DEA/NO-treated B. burgdorferi show S-nitrosylation of proteins but lack tyrosine nitration.

A. B. burgdorferi cells were cultured in the presence or absence of 2.5 mM diethylamine, 0.5 mM DEA/NO or 2.5 mM DEA/NO for 4 h. The biotin-switch technique was used to label S-nitrosylated proteins, which were detected by immunoblot.

B. Immunoblot for nitrotyrosine (Tyr-NO₂) was performed on cell lysates of B. burgdorferi cultures exposed to various concentrations of DEA/NO and authentic ONOO^- for 4 h.