Fig. 7.

BB0445, BosR and NapA are targets of RNS.

A. Aldolase activity was measured from B. burgdorferi crude cell lysates exposed to various concentrations of the zinc-chelator TPEN for 10 min.

B. Aldolase activity of crude cell lysates generated from B. burgdorferi cells treated with 0, 0.5, 1.25 or 2.5 mM DEA/NO for 30 min.

Aldolase activity is represented by the mean consumption of NADH (nmol) per mg protein ± SD of four biological samples.

C. S-nitrosylation of BosR and NapA in B. burgdorferi cells exposed to 0, 0.5 or 2.5 mM DEA/NO for 4 h is shown for separate Western blots using either α-BosR or α-NapA polyclonal antibodies against the affinity-purified biotinylated proteins generated using the biotin-switch method shown in Fig. 5A.