Table 2.
Strains, plasmids and primers
| Strains | Reference | |
|---|---|---|
| Borrelia burgdorferi B31 A3 | Elias et al. (2002) | |
| Borrelia burgdorferi B31 A3 ΔuvrC::aadA | This study | |
| Borrelia burgdorferi B31 A3 ΔuvrB::aadA | This study | |
| Escherichia coli TA4315 (ahpCF-) | Storz et al. (1989) |
| Plasmids | Reference | |
|---|---|---|
| pKFSS1 | Frank et al. (2003) | |
| pPCR-Script Cam SK(+) | Agilent | |
| pCm::ΔuvrC::aadA | This study | |
| pCm::ΔuvrB::aadA | This study |
| Primer name | Sequence (5′–3′) | Reference |
|---|---|---|
| aadA-ClaI-F | ACTGTAATCGATTACCCGAGCTTCAAGGAA | This study |
| aadA-SacII-R | ACTGTACCGCCGTATTTGCCGACTACCTTG | |
| uvrC-KpnI-F | ACTGTAGGTACCAGCACTACTAACCTCTTC | |
| uvrC-ClaI-R | ACTGTAATCGATTCTTTCATAATGTTAGGCAG | This study |
| uvrC-SacII-F | ACTGTACCGCGGTTGCAATAAAAGAGAACTCC | This study |
| uvrC-SacI-R | ACTGTAGAGCTCAAGATATGACTCAGTCTTTG | This study |
| uvrB-KpnI-F | ACTGTAGGTACCTGTTGCAAGGGCAATGTCAC | |
| uvrB-ClaI-R | ACTGTAATCGATTCTCTTTTATTGCCTTAGGC | This study |
| uvrB-SacII-F | ACTGTACCGCGGTGTCAGAGGAGCAAAAGAAC | This study |
| uvrB-SacI-R | ACTGTAGAGCTCAGCCACAGACCAAGATGAAC | This study |
The underlined sequence denotes the restriction endonuclease site of the primer.