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. 2011 Jul;191(2):349–359. doi: 10.1111/j.1469-8137.2011.03711.x

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Copyright © 2011 New Phytologist Trust

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Fig. 1 — Nucleoside hydrolase activities and localization of nucleoside hydrolase (URH)-GFP fusion proteins. (a) Total activity of nucleoside to nucleobase conversion in soluble fractions of extracts from 10-wk-old Arabidopsis thaliana plants. Uridine hydrolase, black bars; inosine hydrolase, pale gray bars; xanthosine hydrolase, dark gray bars. Data are expressed in pkat per mg total soluble protein and show mean ± SD (n = 3). Substrate concentrations correspond to the respective K_M values (Michaelis constant) determined in A. thaliana leaf material with 200 μM uridine, 200 μM inosine, and 60 μM xanthosine. (b) Subcellular localization of URH1-GFP and URH2-GFP in the cytosol of transiently transformed A. thaliana protoplasts.