Table 1.
Metabolite content in roots and leaves of Arabidopsis thaliana wild-type and mutant plants
| Metabolite content (nmol g−1 fresh weight) | |||||||
|---|---|---|---|---|---|---|---|
| URH | urh1 | urh2 | urh1urh2 | ||||
| Root | Leaf | Root | Leaf | Root | Leaf | Root | |
| Uracil | 1.0 ± 0.4 | 0.9 ± 0.5 | 1.5 ± 0.3 | 1.6 ± 0.3 | 1.1 ± 0.2 | 1.2 ± 0.1 | 3.8 ± 3.7 |
| Uridine | 4.6 ± 1.9 | 5.1 ± 2.5 | 223 ± 41* | 16.8 ± 4.6* | 4.3 ± 1.0 | 10.1 ± 4.9 | 191 ± 49* |
| UMP | 10.3 ± 5.1 | 11.4 ± 2.3 | 14.4 ± 5.9 | 8.4 ± 1.9 | 10.5 ± 4.9 | 6.9 ± 4.0 | 21.1 ± 8.3 |
| UDP | 7.1 ± 3.1 | 50.7 ± 15.9 | 17.9 ± 3.7* | 25.0 ± 2.7 | 6.9 ± 1.6 | 33.9 ± 4.6 | 13.5 ± 5.5 |
| UTP | 10.5 ± 1.6 | 13.5 ± 5.6 | 53.5 ± 2.2* | 8.4 ± 1.1 | 11.15 ± 2.8 | 11.0 ± 1.6 | 38.8 ± 8.1* |
| UDPG | 22.6 ± 23.1 | 45.8 ± 18.2 | 135 ± 37* | 86.7 ± 4.5* | 20.7 ± 11.3 | 79.6 ± 3.4 | 186 ± 7.7* |
| Hypoxanthine | – | – | – | – | – | – | – |
| Inosine | – | – | – | – | – | – | – |
| IMP | – | – | – | – | – | – | – |
| Xanthine | – | nd | – | nd | – | nd | – |
| Xanthosine | – | – | 576 ± 194* | 36.1 ± 7.9* | – | – | 857 ± 232* |
| XMP | – | – | – | – | – | – | – |
Metabolites were determined in extracts from 10-wk-old soil-grown A. thaliana plants. Data show mean ± SD (n = 3). Wild-type (URH) and mutant plants were grown in a randomized plot at the same time. Significant differences (P < 0.05), as determined using unpaired two-tailed t-tests, are marked with an asterisk. –, metabolites below the detection limit; nd, not determined because of insufficient separation from another unknown substance; URH, nucleoside hydrolase.