Table 2.
Nucleoside hydrolase activity (pkat g−1 fresh weight) | |||||
---|---|---|---|---|---|
Uridine | Inosine | Xanthosine | Adenosine | Guanosine | |
URH | 2.35 ± 0.56 | 344.79 ± 43.19 | 23.22 ± 0.60 | 47.78 ± 15.67 | 214.96 ± 15.60 |
urh1 | 0.45 ± 0.44* | 519.49 ± 150.89 | 13.51 ± 6.59* | 154.42 ± 57.55* | 236.17 ± 22.68 |
urh2 | 2.85 | 525.68 ± 157.47 | 13.97 ± 9.13 | 100.94 ± 36.47 | 200.78 ± 16.72 |
Activities were determined in pellets of extracts from 10-wk-old soil-grown A. thaliana plants. Data show mean ± SD (n = 3). Wild-type (URH) and mutant plants were grown in a randomized plot at the same time. Significant differences (P < 0.05), as determined using unpaired two-tailed t-tests, are marked with an asterisk. URH, nucleoside hydrolase.