Figure 3.

HPV16E6-mediated upregulation of c-fos in SiHa cells. (a) The mRNA levels of HPV16E6 were detected by RT-PCR after HPV16E6 siRNA transfection into SiHa cells. Cells were transfected with scrambled siRNA or HPV16E6 siRNA for 24 hours. (b) HPV16E6-mediated upregulation of c-fos expression in SiHa cells after H₂O₂ exposure. The transfected cells were exposed to H₂O₂ (1 mM) and harvested at the indicated time points, before being subjected to Western blotting analysis. Representative blots are shown and include β-actin as a loading control, along with the results of densitometric analysis (4 h; normalized to β-actin). *P < 0.05, n = 4. (c) HPV16E6-mediated upregulation of c-fos expression in SiHa cells after TGF-α exposure. Transfected cells were exposed to TGF-α (100 ng/mL) and harvested at the indicated time points, before being subjected to Western blotting analysis. Representative blots are shown and include β-actin as a loading control, along with the results of densitometric analysis (4 h; normalized to β-actin). *P < 0.05, n = 4. (d) HPV16E6-mediated upregulation of c-fos expression in Caski cells after H₂O₂ exposure. The transfected cells were exposed to H₂O₂ (1 mM) and harvested at the indicated time points, before being subjected to Western blotting analysis.