TABLE 2 .

CrdA~P stability is significantly reduced in the presence of CrdS_soluble and CheA3^a

Protein(s)	Half-life (min)
CrdA~P	53.5 ± 6.3
CrdA~P and CrdS	0.7 ± 0.3
CrdA~P and CheA3	8.8 ± 1.3
CrdA~P and CheA2	53.3 ± 9.8
CrdA~P and HK_1190	50.0 ± 0.7
CrdA~P and HK_4262	44.5 ± 6.5

^a Half-lives were determined as indicated in Materials and Methods. Values given are the means ± standard errors. CrdA~³²P was generated by incubation of CrdA with radiolabeled acetyl phosphate. Free acetyl phosphate was removed, and CrdA was incubated with equal molar amounts of each target protein. The decrease in the CrdA~P half-life indicates that both CrdS and CheA3 possess phosphatase activity.