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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Integr Biol (Camb). 2010 Aug 20;2(9):435–442. doi: 10.1039/c0ib00034e

Fig. 4. TRPV4 channels mediate rapid force-induced calcium signaling.

Fig. 4

(A) Phase contrast (left) and fluorescence (right) views of a bound RGD-bead on a cell transfected with TRPV4-EGFP. (B) Calcium increases induced by force application (850 and 2000 pN) through RGD coated microbeads to CHO cells transfected with indicated DNA. (C) Effects of the SA channel blocker GSMTx4 and RRed on calcium influx stimulated by force application through β1 integrin (12G10 antibody). (D) Changes of intracellular Ca2+ induced by the TRPV4 activator PDD (black arrow) in BCE cells transfected with TRPV4, TRPV2 or sham siRNAs. (E) Inhibition of increases in Ca2+ induced by force application to β1 integrin (12G10 antibody) by treatment of cells with TRPV4 siRNA, but not TRPV2 or sham siRNAs.