Figure 1. Influence of Fluoxetine and Desipramine on co-stimulatory molecules expressed on DC.

DCs were developed from bone marrow cells by incubation ex vivo in the presence of GM-CSF for 7 days. The developed DCs were treated with or without Fluoxetine or Desipramine for 24 hr. The MHC-class-II and co-stimulatory molecules expression pattern on CD11c+ DCs were monitored using flow cytometry. Without separation of CD11c+ cells using MACS beads, whole bone marrow cell culture containing developed DCs was subjected to flow cytometry. CD11c+ cells were labeled by PE, while remaining cell markers, MHC-class-II, PD-L1, CD80, CD86, and ICOS-L, were labeled with FITC. # shows the non-stained control Bone marrow cells. The open histogram indicates the FITC staining of PE positive (CD11c+) DCs incubated in medium alone. The solid histograms display the FITC staining of PE positive (CD 11c+) DCs stimulated with Fluoxetine (upper panel) or Desipramine (bottom panel), respectively. C: no drug treated control group. F: Fluoxetine treated group. D: Desipramine treated group. The arrows (L and H) shown in CD86-stained cells indicate CD86^Low and CD86^High populations, respectively.