Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: FEMS Immunol Med Microbiol. 2011 Jun 16;62(3):283–294. doi: 10.1111/j.1574-695X.2011.00816.x

Figure 2. The effects of Fluoxetine and Desipramine on the bacteria (Aa)-antigen-reactive T cell (×Aa-T) responses induced by DCs.

Figure 2

(A and B) The effects of Fluoxetine and Desipramine on the Aa-antigen-specific ×Aa-T cell proliferation induced by co-culture with bone marrow derived DCs (treated with MMC) and Aa-antigen were examined. The lymph node T cells isolated from control non-immunized mice or Aa-immunized mice (4 × 105 cells/well, respectively) were co-cultured with DCs (2 × 104 cells/well, pretreated with MMC) in the presence or absence of Aa-antigen (2 × 106 fixed bacteria/well) for 4 days. Proliferation of T cells was determined by adding [3H] thymidine (0.5 μCi) to each well during the last 16 hours of a total 4 day culture (A). Culture supernatants were collected 3 days after co-culture for the measurement of TNF-α production by ELISA (B). Results are expressed as the mean ± SD of incorporated [3H] thymidine (cpm) or concentration of TNF-α (pg/mL). One representative result from three different experiments is shown. #, significantly higher than non-immunized mice by Student t test (P < 0.01). *, significantly lower than control cultured with Aa by Student t test (P < 0.01).