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Measurement of [14C]-betaine uptake rates in proteoliposomes. (A) Representative uptake of [14C]-betaine in proteoliposomes with wild-type BetP or the mutant BetP-W101A/T351A at 400 mosml/kg. (B) The wild-type BetP activation profile is compared with those of BetPΔC, BetP-W101A/T351A, BetP-W101A/F345A/T351A, BetPΔC-W101A/T351A and BetPΔC-W101A/F345A/T351A. Each value represents the average of three independent measurements. Error bars indicate the standard deviation.
