Table 1. Freeze-fracture particle analysis of BetP wild-type, BetP-W101A/T351A and BetPΔC-W101A/T351A.
Density (particles/μm2) | Diameter (nm)* | Area (nm2)‡ | |
---|---|---|---|
BetP WT | 368.0±15.0 | 10.0±0.8 | 78.5±0.5 |
BetP-W101A/T351A | 331.0±19.0 | 5.1±0.7 | 20.4±0.4 |
BetPΔC-W101A/T351A | 295.0±12.0 | 5.0±0.7 | 19.6±0.4 |
*Diameters were corrected for the thickness of the platinum–carbon film by subtracting 2.5±0.8 nm from the mean diameters obtained from the frequency histograms; ‡Cross-sectional area was obtained by assuming a circular geometry for the particles. WT, wild type. |