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. 2011 Jul 8;12(8):785–796. doi: 10.1038/embor.2011.139

Table 1. Key phosphorylation sites in isoforms of protein kinase D and selected protein kinase D effectors.

Protein Phosphorylation site Function Reference
PKD1 741GEKSFRRSVVG751 A-loop, activation Rozengurt et al, 2005
  913ERVSIL918 Activation, reduce PDZ binding  
DKF-1 581PESQFRKTVVG591 A-loop, activation Feng et al, 2006
DKF-2A 922GEKSFRRSVVG932 A-loop, activation Feng et al, 2007
PKD1 466SRYYKEI472 Conformational change Storz & Toker, 2003
PKD1 90CGFYGLY96 PKCδ binding site Doppler & Storz, 2007
SSH1L 973LKRSHSLA980 Inactivation Eiseler et al, 2009b
Cortactin 293LAKHESQQ300 Inhibit F-actin remodelling Eiseler et al, 2010
SNAIL 6LVRKPSDP13 Transcription de-repression, 14-3-3 binding Du et al, 2010
RIN1 287LRRESSVG294 Inhibit F-actin remodelling Ziegler et al, 2011
PI4KIIIβ 289LKRTASEP296 Activation; PI4P synthesis Hausser et al, 2005
CERT 127LRRHGSMV134 Inhibition of docking with PI4P Fugmann et al, 2007
OSBP 235LQRSLSEL242 Inhibition of docking with PI4P Nhek et al, 2010
KI220 914ITRQMSFD921 Not determined Iglesias et al, 2000
Par-1b 395VQRSVSAN402 Dissociation from membranes Watkins et al, 2008
CREB 128LSRRPSYR135 Transcription activation Johannessen et al, 2007
HSP27 77LSRQLSSG84 Pro-survival chaperone activity, actin stabilization Doppler et al, 2005
DKAR* 408LSRQLTAA415 Change in FRET signal Kunkel et al, 2009
HDAC5 254LRKTASEP261 Transcription de-repression, 14-3-3 binding Vega et al, 2004
  493LSRTQSSP500    
HDAC7 150LRKTVSEP157176LLRKESAP183316LSRTRSEP323444LSRAQSSP451 Transcription de-repression, 14-3-3 binding Dequiedt et al, 2005

Phosphorylated amino acids are shown in bold.

*DKAR is a genetically engineered, non-endogenous FRET-reporter PKD substrate. Due to space limitations, studies on PKD-mediated phosphorylation of Hsp27, CREB and Par-1b were not included in this Review; readers are encouraged to consult the cited references for further information. CERT, ceramide transfer protein; DKF, D-kinase family, Caenorhabditis elegans PKD; HDAC, histone deacetylase; HSP, heat shock protein ; OSBP, oxysterol-binding protein; Par, partitioning defective; RIN1, Ras and Rab interactor 1; SSH1L, Slingshot 1L protein phosphatase.

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