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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Biotechniques. 2010 Jan;48(1):47–52. doi: 10.2144/000113317

Figure 1. Schematic illustration of the procedure for the fabrication of micropatterned PDMS stencils.

Figure 1

The silicon wafer substrate with patterned SU-8 photoresist was covered with PDMS prepolymer. A polyacetate film was placed on the PDMS and clamped with an aluminum block. After curing, the microfabricated PDMS stencils were peeled off from the master, cut into 10 cm2 pieces to fit into a 35 mm tissue culture dish.