Table 1.
Comparison of different ferret studies
| Parameter | Value or description |
||
|---|---|---|---|
| Ferret study 1 | Ferret study 2 | Ferret study 3 | |
| No. of ferrets/arm | 9–10 | 6 | 6 |
| No. of doses | 2 | 1 | 2 |
| HA doses (total HA [μg]) | 7.5, 22.5, 36 | 0.9, 3, 7.5 | 3, 6, 12 |
| Dosing schedule (days) | 1, 28 | 1 | 1, 28 |
| Vaccine vol (μl) | 500 | 500 | 100, 200, 500 |
| Antigen source | Fluzone and Fluvirin (2007–2008) | Fluzone (2007–2008) | Fluzone (2008–2009) |
| NE adjuvant | 20% W805EC | 20% W805EC | 5, 10, and 20% W805EC |
| Control dose (total HA [μg]) and administration route | 36 i.n. and 45 i.m. | 45 i.m. | 45 i.m. |
| Antibody testing days | 27, 48 | 27 | 27, 48 |
| Study day showing cross-reactivity | 48 (5 antigens) | 27 (5 antigens) | ND |
| Challenge arms (μg of HA) | 7.5 | 0.9, 3, 7.5 | ND |
| Challenge time (days) | 49 | 28 | ND |
| Study days with nasal viral shedding | 50–55 | 29–34 | ND |
| Study day with lung and nasal turbinate viral shedding | 53 | ND | ND |
| Day of euthanasia | 63 | 42 | 48 |