. 2011 Jul;18(7):1067–1076. doi: 10.1128/CVI.05030-11

Table 2.

Lymphocyte proliferative response induced in mice primed and boosted with GK-1 peptide^a

Priming/booster group	Mean ± SD cell proliferation index
	Spleen		MLN		PP
	CD4	CD19	CD4	CD19	CD4	CD19
GK-1/GK-1	4.5 ± 0.2^a	5.6 ± 0.92^a	4.7 ± 0.27^a	2.05 ± 0.3^a	1.75 ± 0.72^a	2.25 ± 0.2^a
BLS-GK-1/GK-1	4.5 ± 0.7^a	5 ± 1.03^a	4.25 ± 0.9^a	2.5 ± 0.72^a	2.25 ± 0.52^a	2 ± 0.52^a
BLS + GK-1/GK-1	5.0 ± 1.01^a	7.2 ± 0.25^a	5.0 ± 0.71^a	4.25 ± 0.1^b	2.0 ± 0.48^a	3 ± 0.12^a

Data represent the cell proliferation indexes of CD4 or CD19 lymphocytes of the different lymphoid organs in three different experiments. The stimulation index was calculated by dividing the percentage of lymphocytes which incorporated BrdU from experimental mice by the percentage of cells from nonimmunized PBS-treated mice. Each experiment was performed with lymphocytes pooled from 3 mice. Proliferation was determined by BrdU incorporation into fresh lymphoid PP, MLN, and spleen cells recovered 24 h after boosting with GK-1 and 6 h after BrdU administration. Cells were costained with anti-BrdU (FITC) and rat anti-mouse CD4 (PE) or rat-anti-mouse CD19 (PE). Different letters in each column indicate significant differences (P < 0.05) determined using the Tukey-Kramer multiple-comparisons test.