Table 1.
HI titers of serum IgG and egg yolk IgY raised against H5N1 viral strain (A/Vietnam/1194/04)
| Sample type and group | Total IgY concn (mg/ml)a | HI titer to strain: |
||
|---|---|---|---|---|
| H5N1 SVA 789/06 | H1N1 A/PR/8/34 | H5N1 (adjusted)b | ||
| Hen serum | ||||
| Adjuvant control | <1:8 | |||
| H5N1 5 μg | 8.0 | 1:1,024 | 1:3,673 | |
| H5N1 10 μg | 13.0 | 1:1,024 | 1:2,260 | |
| H5N1 50 μg | 28.7 | 1:4,096 | 1:4,096 | |
| Egg yolk | ||||
| Adjuvant control | 0.4 | <1:8 | 1:2 | |
| H5N1 5 μg | 0.43 | 1:64 | 1:4,271 | |
| H5N1 10 μg | 0.61 | 1:32 | 1:1,505 | |
| H5N1 50 μg | 0.83 | 1:128 | 1:64 | 1:4,426 |
a
These concentrations were determined in serum and yolk samples taken 2 to 3 weeks postboosting.
b
Adjusted for IgY concentration. The HI titer to H5N1 is adjusted for antibody concentration by normalizing all of the results to the IgG concentration in the serum sample from hens immunized with H5N1 at 50 μg (28.7 mg/ml).