Table 1.
Primary antibodies used in this studya
| Antigen | Antibody clone | Isotype | Working MAb concn (μg/ml)b | Specificity |
|---|---|---|---|---|
| B lymphocyte | BAQ155A | IgG1 | 7 | Total B cells |
| CD5 | B29a | IgG2a | 7 | Activation marker |
| CD25 | CACT116A | IgG1 | 15 | IL-2 receptor |
| CACT108A | IgG2a | 15 | IL-2 receptor | |
| LCTB2A | IgG3 | 15 | IL-2 receptor | |
| CD26 | CACT114A | IgG2b | 15 | Activation marker |
| CD45RO | GC42A1 | IgG1 | 10 | Memory/activation marker |
a
Obtained from VMRD Inc. (Pullman, WA).
b
Antibodies were diluted in PBS with 1% fetal calf serum and 0.04% sodium azide.